Category: Mass Spectrometry
Level: Gold
Author: Caroline Pollard and Helena Rapp Wright
The Impact: <p>Reduces overall acquisition times which leads to less energy usage as well as nitrogen gas consumption.</p>
Details
Optimise the chromatographic method to balance short acquisition times with adequate separation. This looks different for gas and liquid chromatography.
Gas Chromatography:
- Narrower or shorter columns to decrease separation time
- Modify temperature ramping and gas flow rate to speed up separation
- Ensure injection volumes are only what is neccessary to reduce the amount of sample needed meaning analysis will be faster
- Consider a greener carrier gas
Liquid Chromatography:
- Suitable columns with narrower ID, shorter columns and smaller particles
- Optimised solvent gradients
- Optimised solvents and consider greener solvents
